QIAseq FastSelect -5S/16S/23S Kit


Cat. No. / ID: QIAseq FastSelect  

Price : $0 ~ $675,890


Unit


For rapid 5S/16S/23S rRNA removal for RNA-seq library preparation from bacterial RNA samples

  • Pan-bacterial 5S/16S/23S rRNA removal from fragmented or full-length RNA for metatranscriptomics studies
  • Novel RNA removal mechanism that doesn't involve hybrid capture or enzymatic digestion
  • Combine with QIAseq FastSelect –rRNA HMR for ribosomal RNA removal from human, mouse and rat samples for host-microbe studies
  • Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library preparation kits
  • In-silico design predicts blocking of >95% of all 5S, 16S and 23S rRNA database sequences

QIAseq FastSelect –5S/16S/23S Kits use a novel method to remove highly abundant bacterial 5S/16S/23S rRNA from your RNA-seq library. QIAseq FastSelect –5S/16S/23S was specifically designed for complex community microbial samples from soil, water, stool and sludge, which have numerous and diverse bacterial populations. The rRNA coverage provided by this kit is based on SILVA 16S sequences (nearly 600,000 entries), SILVA 23S sequences (nearly 170,000 entries) and 5S rRNA database sequences (over 7200 entries), and in-silico modeling shows coverage of >95% of all known 5S, 16S and 23S rRNA sequences. QIAseq FastSelect –5S/16S/23S has been tested with QIAGEN, Illumina and NEB RNA-seq library kits and is compatible with all major methods of RNA-seq library preparation. The simple 14-minute protocol and bead cleanup integrates seamlessly with all common RNA-seq library kits, and can be used on fragmented or full-length (non-fragmented) RNA.

QIA-335925:Includes 3 tubes of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 24 samples

QIA-335927:Includes 1 tube of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 96 samples

QIA-335929:Includes 4 tubes of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 384 samples