Ni-NTA Spin Kit

Ni-NTA Spin Kit

Cat. No. / ID: Ni-NTA Spin Kit  

Price : $0 ~ $28,120

Unit

For fast, small-scale purification of His-tagged proteins

  • Up to 300 μg His-tagged protein per column in as little as 15 minutes
  • Purification under native and denaturing conditions
  • Up to 95% homogeneity in one step
  • Ready-to-use spin columns for rapid automated or manual processing

Ni-NTA silica combines Ni-NTA with a macroporous silica support material optimized to suppress nonspecific hydrophobic interactions. Ni-NTA Spin Columns (His-protein purification spin columns) in the Ni-NTA Spin Kit and available separately provide Ni-NTA silica in a convenient microspin format for easy preparation of multiple samples in parallel. They provide a simple method for functional screening of engineered proteins, selection of clones expressing full-length translation products and comparison of expression levels. Each spin column can purify up to 300 µg of His-tagged protein. Like all Ni-NTA matrices, Ni-NTA spin columns can be used for one-step protein purification under native or denaturing conditions.

The QIAexpress Ni-NTA Protein Purification System, including Ni-NTA Spin Columns and the Ni-NTA Spin Kit, provides reliable, one-step purification of proteins suitable for any application, including:

  • Structural and functional investigations
  • Crystallization for determination of three-dimensional structure
  • Assays involving protein–protein and protein–DNA interaction
  • Immunization to produce antibodies

50 Ni-NTA Spin Columns, Reagents, Buffers, Collection Tubes, 1 μg Control Expression Plasmid