REPLI-g WTA Single Cell Kit
REPLI-g WTA Single Cell Kit
Cat. No. / ID: REPLI-g WTA Single Cell Kit
Price : $0 ~ $246,100
Unit
For whole transcriptome amplification of total RNA or mRNA from single cells
- Complete transcriptome coverage from just single cells
- Uniform WTA with negligible sequence bias due to MDA technology
- Optimized for use with new technologies, including NGS
- Amplification of total RNA or mRNA-enriched (poly A+) RNA
- Novel tool for cancer and stem cell research
The REPLI-g WTA Single Cell Kit enables reliable investigation of effects on transcription regulation at the single-cell transcriptome level and allows uniform amplification of all transcripts from just single cells (1–1000 cells). Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method. The innovative lysis buffer effectively stabilizes cellular RNA, ensuring the resulting RNA accurately reflects the in vivo gene expression profile. All enzymatic steps have been developed to enable efficient processing of RNA for accurate amplification of cDNA, which is achieved with negligible sequence bias using innovative Multiple Displacement Amplification (MDA) technology.
The REPLI-g WTA Single Cell Kit allows uniform amplification of all transcripts from very small samples, accurately representing the transcription pattern of a single cell with very limited, or no, amplification bias. Depending on the protocol, amplified cDNA is highly suited for use in next-generation sequencing (RNA-Seq), gene expression arrays, or for quantitative PCR analysis.
REPLI-g SensiPhi DNA Polymerase, Buffers, and Reagents
QIA-150063：for 24 x 60 µl whole transcriptome amplification reactions (typical yield: 20 µg)
QIA-150065：for 96 x 60 µl whole transcriptome amplification reactions (typical yield: 20 µg)