REPLI-g UltraFast Mini Kit

Cat. No. / ID: REPLI-g UltraFast Mini Kit

Price : $0 ~ $44,500

Unit

For highly uniform whole genome amplification from 10ng of genomic DNA

Amplified DNA can be used in NGS, microarray and PCR applications
Isothermal multiple displacement amplification (MDA) with proof-reading DNA polymerase
Whole genome amplification (WGA) yields 7 to 40 µg amplified DNA from 10 ng of DNA
Ultrafast format for whole genome amplification in 1–1.5 hours

Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). REPLI‑g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. QIAGEN REPLI-g Kits are available in several different sizes and configurations which allows researchers to build the best workflow for their application and lab space. Typical REPLI-g yields range from approx. 7 µg to 40 µg of amplified DNA, depending on which kit and workflow is used.

The REPLI-g UltraFast Kit enables highly uniform and accurate whole genome amplification in just 60–90 minutes.

REPLI-g amplified genomic can be used in a variety of downstream applications, including:

SNP genotyping with TaqMan^® primer/probe sets
qPCR- and PCR-based mutation detection
Next-generation sequencing
STR/microsatellite analysis
Sanger sequencing
RFLP and Southern blot analysis
Array technologies, such as comparative genomic hybridization

DNA Polymerase, Buffers, and Reagents
QIA-150033：for 25 x 20 μl ultrafast whole genome amplification reactions
QIA-150035：for 100 x 20 μl ultrafast whole genome amplification reactions

REPLI-g UltraFast Mini Kit