REPLI-g Single Cell Kit

REPLI-g Single Cell Kit

Cat. No. / ID: REPLI-g Single Cell Kit  

Price : $0 ~ $116,000

Unit

For highly uniform whole genome amplification (WGA) from single cells or limited sample material

  • WGA from single cell material with complete genome coverage
  • Unbiased amplification of genomic loci due to MDA technology
  • Optimized for use with new technologies, including NGS
  • Consistent yields of up to 40 µg (average product length >10 kb)
  • Novel tool for cancer research, stem cell research, or metagenomics

DNA sequence analysis and genotyping of biological samples using innovative instrumentation, such as next-generation sequencing (NGS) platforms, is often limited by the small amount of sample available. The REPLI-g Single Cell Kit is specially designed to uniformly amplify genomic DNA from single cells (1 to <1000 bacterial or tumor cells) or purified genomic DNA with complete genome coverage. Additional protocols are also available for use with fresh or dried blood or fresh or frozen tissue. Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to avoid amplification of contaminating DNA, ensuring highly reliable results every time. Accurate amplification of genomes with negligible sequence bias and no genomic drop-outs is achieved with innovative Multiple Displacement Amplification (MDA) technology. In contrast to PCR-based WGA technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

DNA amplified with the REPLI-g Single Cell Kit can be used in a variety downstream applications, including:

  • Next-generation sequencing
  • SNP genotyping with TaqMan primer/probe sets
  • qPCR- and PCR-based mutation detection
  • STR/microsatellite analysis
  • Sanger sequencing
  • Pyrosequencing
  • Array technologies, such as comparative genomic hybridization (aCGH)

REPLI-g sc Polymerase, Buffers, and Reagents 
QIA-150343：for 24 whole genome amplification reactions (yields up to 40 µg/reaction)
QIA-150345：for 96 whole genome amplification reactions (yields up to 40 µg/reaction)