REPLI-g Kit

REPLI-g Kit

Cat. No. / ID: REPLI-g Kit  

Price : $0 ~ $122,600

Size
Unit

For highly uniform whole genome amplification from 10ng of genomic DNA

  • Amplified DNA can be used in NGS, microarray and PCR applications
  • Isothermal multiple displacement amplification (MDA) with proof-reading DNA polymerase
  • Whole genome amplification (WGA) yields 7 to 40 µg amplified DNA from 10 ng of DNA

Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). REPLI‑g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. QIAGEN REPLI-g Kits are available in several different sizes and configurations which allows researchers to build the best workflow for their application and lab space. Typical REPLI-g yields range from approx. 7 µg to 40 µg of amplified DNA, depending on which kit and workflow is used.

The REPLI-g Mini and Midi Kits offer a single tube workflow with higher yields of amplified DNA.

REPLI-g amplified genomic can be used in a variety of downstream applications, including:

  • SNP genotyping with TaqMan® primer/probe sets
  • qPCR- and PCR-based mutation detection
  • Next-generation sequencing
  • STR/microsatellite analysis
  • Sanger sequencing
  • RFLP and Southern blot analysis
  • Array technologies, such as comparative genomic hybridization

DNA Polymerase, Buffers, and Reagents 
QIA-150023： for 25 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)
QIA-150025： for 100 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)
QIA-150043： for 25 x 50 µl whole genome amplification reactions (typical yield 40 µg per reaction)
QIA-150045： for 100 x 50 µl whole genome amplification reactions (typical yield 40 µg per reaction)