REPLI-g FFPE Kit

REPLI-g FFPE Kit

Cat. No. / ID: REPLI-g FFPE Kit  

Price : $0 ~ $109,700

Unit

For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue

  • Whole genome amplification directly from paraffin sections
  • Whole genome amplification of purified DNA from FFPE tissue
  • Scalable and standardized DNA yields: up to 40 µg per tissue section
  • Fast and easy protocol for rapid results

A lack of sufficient quantities of genomic DNA for genomic analysis can be overcome by global amplification of all DNA within a sample (whole genome amplification). The REPLI-g FFPE Kit consists of DNA Polymerase, novel buffers, and reagents that enable efficient whole genome amplification from formalin-fixed and paraffin embedded (FFPE) tissue without the requirement for prior DNA purification. After lysis of the tissue section, the DNA is processed so that fragmented DNA is ligated. The long DNA strands created are amplified using proven REPLI-g technology.

DNA amplified using the REPLI-g FFPE procedure is highly suited for use in:

  • Real-time PCR
  • End-point PCR
  • SNP genotyping
  • Microsatellite analysis

DNA Polymerase, Buffers, and Reagents 
QIA-150243：for 25 x 50 μl whole genome amplification reactions
QIA-150245：for 100 x 50 μl whole genome amplification reactions