REPLI-g Advanced DNA Single Cell Kit

Cat. No. / ID: REPLI-g Advanced DNA Single Cell Kit

Price : $0 ~ $116,000

Unit

For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA

WGA with complete genome coverage from single eukaryotic cells or 1 ng genomic DNA
Simple 3-step protocol requires only 2 hours and 20 minutes, start to finish
Consistent yields of up to 25–35 µg DNA from 1 eukaryotic cell
Includes cryo-protect reagent to minimize DNA damage during single cell capture, storage or shipping

DNA sequence analysis and genotyping of biological samples using next-generation sequencing (NGS) platforms is often limited by the amount of available sample. The REPLI-g Advanced DNA Single Cell Kit has been specifically optimized for use in sensitive microarray and NGS applications, providing high uniformity, with minimal allelic dropout, when starting with single cells or purified genomic DNA. Buffers and reagents undergo a unique, controlled decontamination procedure to minimize contaminating DNA and ensure highly reliable results. Accurate amplification of genomes with negligible sequence bias and minimal genomic dropouts is achieved with innovative Multiple Displacement Amplification (MDA) technology.

DNA amplified with the REPLI-g Advanced DNA Single Cell Kit can be used in a variety downstream applications, including:

Whole genome sequencing
Exome sequencing
SNP genotyping arrays
Array CGH
qPCR/PCR technologies
Sanger sequenc
Pyrosequencing

Buffers, enzymes and single cell storage solution
QIA-150363：for 24 WGA reactions
QIA-150365：for 96 WGA reactions

REPLI-g Advanced DNA Single Cell Kit