EpiTect Fast FFPE Bisulfite Kit

EpiTect Fast FFPE Bisulfite Kit

Cat. No. / ID: EpiTect Fast FFPE Bisulfite Kit  

Price : $0 ~ $86,800

Size

For ultrafast, highly efficient bisulfite conversion from FFPE samples in spin-column

  • Optimized procedure enables high cytosine conversion rates of over 99%
  • Efficient bisulfite conversion in as little as 30 minutes
  • Unique DNA Protect technology prevents DNA degradation
  • Fast, streamlined procedure for efficient conversion and purification of DNA, also in 96-well plates
  • Dedicated solutions for FFPE slices

Formalin-fixed, paraffin-embedded (FFPE) tissues are processed with the EpiTect Fast FFPE Bisulfite Kit, which consists of the EpiTect Fast FFPE Lysis Kit, containing specialized buffers for efficient deparaffinization and lysis of FFPE tissue slices, and the EpiTect Fast DNA Bisulfite Kit for fast and effective bisulfite conversion of the extracted DNA. The protocol includes an optimized step to facilitate binding of DNA and can be used with single slices of FFPE tissue (10 µm in thickness). The DNA fragmentation that inevitably occurs when DNA is extracted from FFPE tissues is made worse by the harsh conditions of subsequent bisulfite treatment. QIAGEN's reliable DNA Protect technology minimizes fragmentation during the conversion reaction, resulting in superior yields of bisulfite converted DNA.

The EpiTect Fast Bisulfite Kit delivers fast and efficient bisulfite conversion of DNA that is highly suited for all techniques currently used for the analysis of DNA methylation, including PCR (end-point, real-time and methylation-specific), bisulfite sequencing (direct and cloning), COBRA (Combined Bisulfite Restriction Analysis) and pyrosequencing.

QIA-59844：For 50 preps; Deparaffinization Solution, Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

QIA-59740：For 96-well format; Deparaffinization Solution, Lysis Buffer, Proteinase K, 2 x EpiTect 96-well Plates, Bisulfite Solution, DNA Protect Buffer, Carrier RNA, Buffers