RNA Spike-In Kit, For RT
RNA Spike-In Kit, For RT
Cat. No. / ID: QIA-339390
Price : $7,800
For quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR experiments
- An easy and convenient control of your RNA samples and RT reactions
- Save time and reagents by identifying poor-quality samples early
- Available in an easy-to-use, pre-mixed format
- Highly flexible – use only the spike-ins you need for your study
The RNA Spike-In Kit enables quality control of the RNA isolation, cDNA synthesis and PCR amplification steps of miRCURY LNA miRNA PCR or miRCURY LNA miRNA Probe PCR experiments. Identifying any poor-quality samples early in the process saves you time and reagents. Plus, the kit is easy to use and comes in a premixed format that allows you to use only the spike-ins you need for your analyses.
Our RNA spike-ins (UniSp2, UniSp4, UniSp5, UniSp6) resemble miRNAs in structure but lack close sequence similarities to known miRNAs. The kit also includes a synthetic version of the C. elegans miRNA, cel-miR-39-3p. The RNA Spike-In Kit is distributed in two vials, each vial contains RNA sufficient for 50 reactions. Also included is MS2 carrier RNA, which improves the stability of the RNA spike-ins without interfering with downstream reactions.
| Reagent | Amount | Conc. (resuspended) | |
| Vial 1 | Synthetic UniSp2 RNA (22 nt) | 160 fmol | 2 fmole/µl |
| Synthetic UniSp4 RNA (22 nt) | 1.6 fmol | 0.02 fmole/µl | |
| Synthetic UniSp5 RNA (22 nt) | 0.016 fmol | 0.00002 fmole/µl | |
| MS2 total RNA | 50 ng | 0.625 ng/µl | |
| Vial 2 | Synthetic cel-miR-39-3p RNA | 0.16 fmol | 0.002 fmole/µl |
| MS2 total RNA | 50 ng | 0.625 ng/µl |
Quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR and miRCURY LNA miRNA Probe PCR experiments.
Contains the UniSp2, UniSp4, and UniSp5 RNA Spike-in Template Mix and the cel-miR-39-3p RNA Spike-in Template