QuantiTect Multiplex PCR Kit

QuantiTect Multiplex PCR Kit

Cat. No. / ID: QuantiTect Multiplex PCR Kit  

Price : $0 ~ $114,230

Unit

For multiplex qPCR and two-step multiplex qRT-PCR using sequence-specific probes

  • Highly sensitive detection of low-copy targets
  • qPCR using sequence-specific probes or SYBR Green
  • Accurate quantification over several logs of template
  • No need to optimize reaction and cycling conditions
  • Detection of up to 5 targets in the same tube

QuantiTect PCR Kits (qPCR kits) enable sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes or SYBR Green I detection. The real-time PCR kits also allow reliable quantification of up to 5 gDNA or cDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization.

The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mixes in QuantiTect PCR Kits can be stored at 2–8°C. Two kit formats are available for multiplex PCR using sequence-specific probes: the QuantiTect Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiTect Multiplex PCR NoROX Kit for all other cyclers.

ROX dye Kit Compatible cyclers
Supplied in master mix QuantiTect Multiplex PCR Kit Cyclers from Applied Biosystems
Absent from master mix QuantiTect Multiplex PCR NoROX Kit Rotor-Gene cyclers, and cyclers from Bio-Rad, Cepheid, Eppendorf, Roche, Agilent, and other suppliers

 

QuantiTect PCR Kits can be used for gene expression analysis of cDNA or quantification of gDNA on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent. 

QIA-204543：For 200 reactions: 3 x 1.7 ml 2x QuantiTect Multiplex PCR Master Mix (with ROX dye), 2 x 2 ml RNase-Free Water

QIA-204545：For 1000 reactions: 25 ml 2x QuantiTect Multiplex PCR Master Mix (with ROX dye), 20 ml RNase-Free Water