QuantiNova SYBR Green RT-PCR Kit

QuantiNova SYBR Green RT-PCR Kit

Cat. No. / ID: QuantiNova SYBR Green RT-PCR Kit  

Price : $0 ~ $140,100

Unit

For highly sensitive and specific one-step qPCR using SYBR® Green I for gene expression analysis

  • Unique two-phase hot-start procedure for room-temperature setup
  • Visual pipetting control, resulting in fewer pipetting errors
  • Internal control for positive in-process verification of successful RT-PCR
  • Accurate quantification over several logs of template
  • Sensitive detection of up to 5 targets in 1 tube

QuantiNova RT-PCR Kits (real-time RT-PCR kits) enable sensitive quantification of RNA targets by real-time one-step PCR using SYBR Green I or sequence-specific probes. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. The combination of a unique two-phase hot-start and PCR buffer system in the ready-to-use master mix allows room-temperature setup and ensures highly sensitive qRT-PCR on any real-time cycler.

The optional QuantiNova Internal Control RNA can be used to test successful reverse transcription and amplification. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. Furthermore, the visual pipetting control can be used to monitor correct pipetting. The integrated gDNA reduction step in the QuantiNova Probe RT-PCR procedure prevents overquantification of transcripts caused by genomic DNA carryover.

The QuantiNova SYBR Green RT-PCR Kit can be used for gene expression analysis of RNA targets on any real-time cycler. This includes the Rotor-Gene Q and instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.

QIA-208152：For 100 reactions: 1 ml QuantiNova SYBR Green RT-PCR Master Mix, 20 µl QuantiNova SYBR Green RT Mix, 20 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 250 µl ROX Reference Dye, 1.9 µl RNase-Free Water

QIA-208154：For 500 reactions: 3 x 1.7 ml QuantiNova SYBR Green RT-PCR Master Mix, 100 µl QuantiNova SYBR Green RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water

QIA-208156：For 2500 reactions: 15 x 1.7 ml QuantiNova SYBR Green RT-PCR Master Mix, 5 x 100 µl QuantiNova SYBR Green RT Mix, 2 x 100 µl Internal Control RNA , 5 x 500 µl Yellow Template Dilution buffer, 5 x 1 ml ROX Reference Dye, 10 x 1.9 µl RNase-Free Water