QuantiNova Multiplex RT-PCR Kit

QuantiNova Multiplex RT-PCR Kit

Cat. No. / ID: QuantiNova Multiplex RT-PCR Kit  

Price : $0 ~ $193,900

Unit

For highly sensitive and specific one-step multiplex qRT-PCR using sequence-specific probes 

  • Unique two-phase hot-start procedure for room-temperature setup
  • Visual pipetting control, resulting in fewer pipetting errors
  • Internal control for positive in-process verification of successful RT-PCR
  • Accurate quantification over several logs of template
  • Sensitive detection of up to 5 targets in 1 tube

The QuantiNova Multiplex RT-PCR Kit enables fast and reliable quantification of up to 5 RNA targets in a single tube by multiplex real-time RT-PCR. Our Q-Bond technology and optimized master mix promote ultrafast multiplex real-time RT-PCR within 1 hour. The combination of a unique two-phase hot-start procedure with our multiplex PCR buffer system ensures highly sensitive qRT-PCR on any real-time cycler without the need for optimization, and enables automated reaction setup at room temperature. The kit also provides protocols for extracted RNA and direct amplification from cultured cells, even down to a single cell. The optional QuantiNova Internal Control RNA can be used to monitor successful reverse transcription and amplification, and the visual pipetting control can be used to monitor correct pipetting.

The QuantiNova Multiplex RT-PCR Kit can be used for multiplex gene expression analysis of RNA targets on any real-time cycler. To fully benefit from multiplexing, we recommend using an instrument that provides up to 5-plex capacity, such as the Rotor-Gene Q.

QIA-208552：For 100 reactions: 0.5 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 20 µl QuantiNova Multiplex RT-Mix, 20 µl QuantiNova IC RNA, 500 µl QuantiNova Yellow Template Dilution Buffer, 250 µl QN ROX Reference Dye, 1.9 ml RNase-Free Water

QIA-208554：For 500 reactions: 2 x 1.3 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 100 µl QuantiNova Multiplex RT-Mix, 100 µl QuantiNova IC RNA, 500 µl QuantiNova Yellow Template Dilution Buffer, 1 ml QN ROX Reference Dye, 4 x 1.9 ml RNase-Free Water

QIA-208556：For 2500 reactions: 10 x 1.3 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 5 x 100 µl QuantiNova Multiplex RT-Mix, 3 x 100 µl QuantiNova IC RNA, 3 x 500 µl QuantiNova Yellow Template Dilution Buffer, 3 x 1 ml QN ROX Reference Dye, 20 x 1.9 ml RNase-Free Water