PowerProtect DNA/RNA
PowerProtect DNA/RNA
Cat. No. / ID: PowerProtect DNA/RNA
Price : $0 ~ $52,340
容積
For stabilization and preservation of the microbial community profile and expression profile in stool samples over time
- Preserves bacterial DNA and RNA in stool samples after collection
- Penetrates cells and halts biological activity
- Maintains the microbial community and functional profile during transport and storage
- Nucleic acid stabilization in room temperature or frozen for an extended period
- Stabilization for shorter duration at 35°C
After sample collection, the stabilization should be carried out as quickly as possible in order to preserve the microbial profile. To stabilize and protect bacterial DNA and RNA in stool samples submerge the material completely in a sufficient volume of PowerProtect DNA/RNA. Use at least 4 volumes of reagent per sample (or approximately 2 ml reagent per 500 mg stool). We recommend using up to 9 volumes of reagent for dry stool samples. Make sure the stabilized sample is stored in a container that is suitable for centrifugation, and which is large enough to also contain the volume of the PBS that will be added after storage, before proceeding with the nucleic acid extraction.
For a complete stabilization, storage and extraction solution, use PowerProtect DNA/RNA with diverse kits from the QIAGEN Power Pro kit line, intended for use with stool samples.
Nucleic acids from stool samples stabilized with PowerProtect DNA/RNA can be used for various applications, depending on the extraction. The DNA can be used immediately in PCR analysis and other downstream applications, including qPCR and NGS. The RNA can be used in downstream applications including RT-PCR, qPCR, digital PCR and next-generation sequencing (e.g., RNA-seq or metatranscriptome).
QIA-14800：500 ml PowerProtect DNA/RNA reagent for ambient temperature stabilization of nucleic acids in stool
QIA-14810：1000 ml PowerProtect DNA/RNA reagent for ambient temperature stabilization of nucleic acids in stool