QuantiTect Multiplex RT-PCR Kit

Cat. No. / ID: QuantiTect Multiplex RT-PCR Kit

Price : $0 ~ $229,890

Unit

For one-step multiplex qRT-PCR using sequence-specific probes for gene expression analysis

Highly sensitive detection of low-copy targets
qRT-PCR using sequence-specific probes or SYBR Green
Accurate quantification over several logs of template
No need to optimize reaction and cycling conditions
Detection of reference gene and up to 3 targets in the same tube

QuantiTect RT-PCR Kits enable sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes or SYBR Green I detection. The kits also allow reliable quantification of up to 5 RNA targets in a single tube by multiplex, real-time one-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qRT-PCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mixes in QuantiTect one-step RT-PCR kits can be stored at 2–8°C.

Two kit formats are available for multiplex RT-PCR using sequence-specific probes: the QuantiTect Multiplex RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiTect Multiplex RT-PCR NoROX Kit for all other cyclers. The QuantiTect SYBR Green RT-PCR Kit is supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts.

ROX dye	Kit	Compatible cyclers
Supplied in master mix	QuantiTect Multiplex RT-PCR Kit	Cyclers from Applied Biosystems
Absent from master mix	QuantiTect Multiplex RT-PCR NR Kit	Rotor-Gene cyclers, and cyclers from Bio-Rad, Cepheid, Eppendorf, Roche, Agilent, and other suppliers

QuantiTect RT-PCR Kits can be used for gene expression analysis of RNA targets on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.

QIA-204643：For 200 reactions: 3 x 1.7 ml 2x QuantiTect Multiplex RT-PCR Master Mix (with ROX dye), 100 µl QuantiTect Multiplex RT Mix, 2 x 2 ml RNase-Free Water

QIA-204645：For 1000 reactions: 25 ml 2x QuantiTect Multiplex RT-PCR Master Mix (with ROX dye), 0.5 ml QuantiTect Multiplex RT Mix, 20 ml RNase-Free Water

QuantiTect Multiplex RT-PCR Kit