QuantiTect Multiplex RT-PCR NR Kit
QuantiTect Multiplex RT-PCR NR Kit
Cat. No. / ID: QuantiTect Multiplex RT-PCR NR Kit
Price : $0 ~ $226,340
Unit
For one-step multiplex qRT-PCR using sequence-specific probes for gene expression analysis
- Highly sensitive detection of low-copy targets
- qRT-PCR using sequence-specific probes or SYBR Green
- Accurate quantification over several logs of template
- No need to optimize reaction and cycling conditions
- Detection of reference gene and up to 3 targets in the same tube
QuantiTect RT-PCR Kits enable sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes or SYBR Green I detection. The kits also allow reliable quantification of up to 5 RNA targets in a single tube by multiplex, real-time one-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qRT-PCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mixes in QuantiTect one-step RT-PCR kits can be stored at 2–8°C.
Two kit formats are available for multiplex RT-PCR using sequence-specific probes: the QuantiTect Multiplex RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiTect Multiplex RT-PCR NoROX Kit for all other cyclers. The QuantiTect SYBR Green RT-PCR Kit is supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts.
| ROX dye | Kit | Compatible cyclers |
| Supplied in master mix | QuantiTect Multiplex RT-PCR Kit | Cyclers from Applied Biosystems |
| Absent from master mix | QuantiTect Multiplex RT-PCR NR Kit | Rotor-Gene cyclers, and cyclers from Bio-Rad, Cepheid, Eppendorf, Roche, Agilent, and other suppliers |
QuantiTect RT-PCR Kits can be used for gene expression analysis of RNA targets on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.
QIA-204843：For 200 reactions: 3 x 1.7 ml 2x QuantiTect Multiplex RT-PCR NoROX Master Mix (without ROX dye), 100 µl QuantiTect Multiplex RT Mix, 2 x 2 ml RNase-Free Water
QIA-204845：For 1000 reactions: 25 ml 2x QuantiTect Multiplex RT-PCR NoROX Master Mix (without ROX dye), 0.5 ml QuantiTect Multiplex RT Mix, 20 ml RNase-Free Water