ECL Detection Kit
ECL Detection Kit
Cat. No. / ID: S1GNM03j20001
Price : $1,450
Unit : 50mL*2
ECL Detection Kit is a horseradish peroxidase (HRP) substrate and it is widely utilized for the identification of specific proteins. As shown in Fig. 1, protein samples are initially separated through SDS-PAGE and then transferred onto either nitrocellulose or polyvinylidene fluoride (PVDF) membranes. Following a blocking step, the membrane undergoes probing with a primary antibody. After a thorough washing procedure, the membrane is incubated with a secondary antibody conjugated to horseradish peroxidase (HRP) enzyme. After a final washing step, the membrane is exposed to the ECL Detection Kit to generate a detectable signal.
Instruction manual：